400-920-2911 sales@csnpharm.cn
您的位置: 首页 / 染料及荧光试剂 / 5(6)-FAM SE
CAS No.: 117548-22-8
5(6)-FAM SE, also called 5(6)-FAM NHS, is consist of two parts, the fluorophore 5(6)-FAM and the reactive group N-Hydroxysuccinimide (NHS).
规格 | 价格 | 库存 |
---|---|---|
1mg | RMB 145 | 产品咨询 |
10mg | RMB 260 | 产品咨询 |
50mg | RMB 539 | 产品咨询 |
100mg | RMB 800 | 产品咨询 |
1g | RMB 6040 | 产品咨询 |
描述
5(6)-FAM SE, also called 5(6)-FAM NHS, is consist of two parts, the fluorophore 5(6)-FAM and the reactive group N-Hydroxysuccinimide (NHS).
保存条件
Store at -20°C in dark
使用方法
Guidance for conjugation of Fluorophore-NHS with protein: Step 1 Solutions & buffers 1 M Sodium Phosphate Buffer, pH 7.0 10x NaH2PO4 1 M 125 ml Na2HPO4 1 M 875 ml Reaction buffer Sodium Phosphate buffer (pH 7.0) 0.1 M 100 ml KOAc(pH 7.4) 75 mM 37.5 ml DTT 2 mM 2 ml Step 2 Make a 20 mM stock of Fluorophore-NHS (e.g. 5(6)-FAM NHS) in DMSO Step 3 Enzyme storage buffer HEPES–KOH (pH 7.4) 20 mM 20 ml KOAc (pH 7.4) 100 mM 50 ml DTT 2 mM 2 ml Glycerol 10% 100 ml Preparation: Pure lyophilized protein can be dissolved in reaction buffer to a final concentration of >2.0 mg ml-1. If the protein is already in solution, it should be brought into reaction buffer by dialyzing overnight at 4°C against 2 l reaction buffer, by gel filtration, or by repeated buffer exchange through a protein concentrator of desired MW
产品功能
5(6)-FAM SE, also called 5(6)-FAM NHS, is consist of two parts, the fluorophore 5(6)-FAM and the reactive group N-Hydroxysuccinimide (NHS). 5(6)-FAM is a mixture of 5-carboxy and 6-carboxy derivatives of fluorescein. When proteins conjugated with 5(6)-FAM NHS through the reaction of NHS with the amine groups, the proteins are labeled with 5(6)-FAM with excitation wavelength of 492 nm and emission wavelength of 514 nm.
联系我们
官方电话:400-920-2911
二维码
微信公众号 CSN官网