CY5-SE, also called CY5-NHS, is consist of two parts, the fluorophore CY5 and the reactive groups N-Hydroxysuccinimide (NHS).

Store at -20°C in dark

Guidance for conjugation of Fluorophore-NHS with protein: Step 1 Solutions & buffers 1 M Sodium Phosphate Buffer, pH 7.0 10x NaH₂PO₄ 1 M 125 ml Na₂HPO₄ 1 M 875 ml Reaction buffer Sodium Phosphate buffer (pH 7.0) 0.1 M 100 ml KOAc(pH 7.4) 75 mM 37.5 ml DTT 2 mM 2 ml Step 2 Make a 20 mM stock of Fluorophore-NHS (e.g. CY5-NHS) in DMSO Step 3 Enzyme storage buffer HEPES–KOH (pH 7.4) 20 mM 20 ml KOAc (pH 7.4) 100 mM 50 ml DTT 2 mM 2 ml Glycerol 10% 100 ml Preparation: Pure lyophilized protein can be dissolved in reaction buffer to a final concentration of >2.0 mg ml^-1. If the protein is already in solution, it should be brought into reaction buffer by dialyzing overnight at 4°C against 2 l reaction buffer, by gel filtration, or by repeated buffer exchange through a protein concentrator of desired MWCO (M

CY5-SE, also called CY5-NHS, is consist of two parts, the fluorophore CY5 and the reactive groups N-Hydroxysuccinimide (NHS). When proteins conjugated with CY5-NHS through the reaction of NHS with the amine group, the proteins are labeled with CY5 with excitation wavelength of 649 nm and emission wavelength of 670 nm.