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CAS No.: 23491-45-4

Hoechst 33258 3HCl

Hoechst 33258 3HCl is a widely used nucleic acid stain which can bind to minor-groove of dsDNA and emit blue fluorescence.

规格 价格 库存
50mg RMB 550 产品咨询
100mg RMB 930 产品咨询
1g RMB 1936 产品咨询

描述

Hoechst 33258 3HCl is a widely used nucleic acid stain which can bind to minor-groove of dsDNA and emit blue fluorescence.

保存条件

The solid could be stored in dark at -20 ℃. The stock solution should be stored at 4°C in the dark for up to 6 months.

使用方法

Make a stock solution of Hoechst 33258 at 1 mg/ml in ddH2O. The stock solution can be stored at 4°C in the dark for up to 6 months For cell suspension 1. Incubate cell suspensions (0.5-2.0 × 106 cells/ml, in PBS) with Hoechst 33258 working solution at 1-10 μg/mL for 10 min. Usually the concentration of working solution at 1 μg/mL is enough (For example, add 1 μL Hoechst 33258 stock solution in 1 mL cell suspension.). Note: Hoechst 33258 can stain nuclei of both live and dead cells. 2. Apply samples to cytospin chambers and centrifuge at 500 rpm for 2 minutes. After air-drying, place glass coverslips over cells to reduce light diffraction (no mounting gel necessary) and observe cells in a fluorescence microscope equipped with a DAPI filter, using a 60X objective.{{Kasibhatla S, Amarante-Mendes GP, Finucane D, Brunner T, Bossy-Wetzel E, Green DR. Staining of suspension cells with hoechst 33258 to detect apoptosis. CSH Protoc. 2006 Aug 1;2006(3):pdb.prot4492. doi: 1

产品功能

Hoechst 33258 3HCl is a widely used nucleic acid stain which can bind to minor-groove of dsDNA and emit blue fluorescence. It is similar to Hoechst 33342. Hoechst 33258 is excited at 352 nm and emits at 461 nm.

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