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CAS No.: 2610-10-8

Direct Red 80/天狼猩红 Catalog No. CSN61897

Synonyms: 天狼猩红;Sirius Red

Direct Red 80 has been used as an ingredient in the preparation of saturated picric acid for immunohistochemical detection and quantitation of collagen in liver tissues.

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描述Direct Red 80 has been used as an ingredient in the preparation of saturated picric acid for immunohistochemical detection and quantitation of collagen in liver tissues.

Sirius Red for Collagen Staining Protocol^[1]
Solutions and Reagents:
Solution A. Picro-sirius red
Direct Red 80 ----------------------------- 0.5 g
Saturated aqueous solution of picric acid ---------500 ml
Add a little solid picric acid to ensure saturation (This is important).
NOTE: This is a dangerous suggestion. Just add only the liquid solution.

Solution B. Acidified water
Add 5 ml acetic acid (glacial) to 1 litre of water (tap or distilled).

Procedure:
1. De-wax and hydrate paraffin sections.
2. (Optional, and not usually done) Stain nuclei with Weigert's haematoxylin (as for the van Gieson method, but more strongly, then wash the slides for 10 minutes in running tap water).
3. Stain in picro-sirius red (Solution A) for one hour (This gives near-equilibrium staining, which does not increase with longer times. Shorter times should not be used, even if the colours look OK.)
4. Wash in two changes of acidified water (Solution B). 5. Physically remove most of the water from the slides by vigorous shaking or (for a few slides only) blotting with damp filter paper.
5. Dehydrate in three changes of 100% ethanol.
6. Clear in xylene and mount in a resinous medium.
参考文献

[1] Sirius Red for Collagen Staining Protocol

实验方案

计算器摩尔计算器总药量计算器工作液计算器
储存液制备
- 1 mg
- 5 mg
- 10 mg
- 1 mM
- 0.7283 mL
- 3.6415 mL
- 7.2829 mL
- 5 mM
- 0.1457 mL
- 0.7283 mL
- 1.4566 mL
- 10 mM
- 0.0728 mL
- 0.3641 mL
- 0.7283 mL

技术信息

CAS号	2610-10-8	储存条件	粉末液体 -20°C:3-6 月 -80°C:12月
分子式	C45H26N10Na6O21S6	运输	蓝冰
分子量	1373.07	别名	天狼猩红;Sirius Red

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