2024548-03-4|Lin28-let-7a Antagonist 1|CsnPharm

CAS No.: 2024548-03-4

Lin28-let-7a antagonist 1 Catalog No. CSN22447

Synonyms: Lin28-let-7a Antagonist 1

Lin28-let-7a antagonist 1 shows a clear antagonistic effect against the Lin28-let-7a interaction with an IC50 of 4.03 μM for Lin28A-let-7a-1 interaction.

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描述MicroRNAs (miRNAs) are small noncoding RNAs that play important roles in posttranscriptional gene regulation. In animal cells, miRNAs regulate their targets by translational inhibition and mRNA destabilization^[1]. Currently, there are over 1000 known human microRNAs, and microRNAs control over 50% of mammalian protein coding genes^[2]. MicroRNAs act to decrease the expression of messenger RNAs that contain stretches of sequence complementary to the microRNA^[3].MiRNA targeting is defined by the complementarities between positions 2-8 of miRNA 5'-end with generally the 3'-untranslated region of target mRNAs (messenger RNAs). The capacity of miRNAs to simultaneously inhibit many different mRNAs allows for an amplification of biological responses^[4].Lin28-let-7a antagonist 1 shows a clear antagonistic effect against the Lin28-let-7a interaction with an IC50 of 4.03 μM for Lin28A-let-7a-1 interaction. Interestingly, Lin28-let-7a antagonist 1 also inhibits the Lin28B-let-7 interaction, with slightly reduced potency. Dicer processing assay reveals that Lin28-let-7a antagonist 1 clearly blocks the formation of the Lin28A-pre-let-7g complex and induces miRNA processing, resulting in the generation of mature let-7g miRNA. When the expression of both Lin28A and Lin28B are knocked-down using siRNA in JAR cells, the Lin28-let-7a antagonist 1-induced increase in cellular let-7 levels is clearly attenuated. Lin28A-let-7a-IN-1 increases mature let-7 levels in PA-1 cells that express high level of Lin28A. On the other hand, let-7 levels are not affected by 1 in MCF7 cells that rarely express Lin28 proteins. Taken together, Lin28-let-7a antagonist 1 specifically induces the increase in the cellular let-7 levels by targeting Lin28^[5].
参考文献

实验方案

计算器摩尔计算器总药量计算器工作液计算器
储存液制备
- 1 mg
- 5 mg
- 10 mg
- 1 mM
- 1.7135 mL
- 8.5676 mL
- 17.1353 mL
- 5 mM
- 0.3427 mL
- 1.7135 mL
- 3.4271 mL
- 10 mM
- 0.1714 mL
- 0.8568 mL
- 1.7135 mL

技术信息

CAS号	2024548-03-4	储存条件	粉末 Sealed in dry,2-8°C 液体 -20°C:3-6 月 -80°C:12月
分子式	C31H29N5O7	运输	蓝冰
分子量	583.59	别名	Lin28-let-7a Antagonist 1

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Lin28-let-7a antagonist 1 Catalog No. CSN22447

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