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CAS No.: 3520-42-1

Sulforhodamine B

The protein dye sulforhodamine B (SRB) could bind electrostatically and pH dependent on protein basic amino acid residues of trichloroacetic acid–fixed cells.

规格 价格 库存
100mg RMB 450 产品咨询
1g RMB 630 产品咨询

描述

The protein dye sulforhodamine B (SRB) could bind electrostatically and pH dependent on protein basic amino acid residues of trichloroacetic acid–fixed cells.

保存条件

Store in dark at room temperature.

使用方法

Preperation: 1. 10% (w/v) TCA (trichloroacetic acid) 2. 1% Acetic acid. 3. 0.4% (w/v) SRB dissolved in 1% acetic acid (store at 4°C protected from light). 4. 10 mM Unbuffered Tris base (pH 10.0). 5. Deionized water. General protocol: 1. Remove gently the growth medium by flipping the culture plate (be cautious; medium contains potentially toxic drugs). 2. Remove residual medium by utilizing a sterile piece of gauze while keeping the culture plate upside down. 3. Fix the cellular protein using 100 µL/well of 10% TCA (4°C), and store the plates at 4°C for at least 1 h prior to analysis by the SRB assay. 4. Rinse TCA using an automated 96-well plate washer and add five washing cycles using deionized water (200 µL/well). 5. Sharply flick the plates over a sink and remove roughly the residual wash solution with a piece of gauze. 6. Pipet 100 µL of SRB solution into each well of the culture plate using a multichannel pipet. Allow a 30-min staining period. 7. Remove the SRB from the culture

产品功能

The protein dye sulforhodamine B (SRB) could bind electrostatically and pH dependent on protein basic amino acid residues of trichloroacetic acid–fixed cells. Under mild acidic conditions SRB binds to and under mild basic conditions it can be extracted from cells and solubilized for measurement. Based on this principle, sulforhodamine B assay was developed to measure drug-induced cytotoxicity and cell proliferation for large-scale drug-screening applications. λex 565 nm; λem 586 nm in H2O

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