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CAS No.: 65162-13-2
1-methoxyPMS is a photochemically stable electron mediator between NAD(P)H and tetrazolium dyes.
规格 | 价格 | 库存 |
---|---|---|
50mg | RMB 630 | 产品咨询 |
100mg | RMB 990 | 产品咨询 |
250mg | RMB 1395 | 产品咨询 |
描述
1-methoxyPMS is a photochemically stable electron mediator between NAD(P)H and tetrazolium dyes.
保存条件
The solid could be stored at room temperature.
使用方法
General protocol for cck-8 assay:
1. Prepare cck-8 working solution: dissolve WST-8 (CSN12409) and 1-mPMS in ddH2O to a final concentration of 500μM and 20μM, respectively.
2. Please ensure that the volume of the culture medium is just 100μL in every well.
Note: For WST-8 can be reduced, make sure that there is no reductant or oxidant in the culture media. If you add compound during cell culture, please wash every well for 2 times with PBS and add fresh culture medium before detection.
3. Add 100μL of cck-8 working solution into every well of 96-well plate.
4. Gently shake the plate to mix the solution well.
5. Incubate the plate in a CO2 incubator at 37°C for 0.5-4h. After incubation, the colour of the solution should turn to orange.
6. Read the plate at 450 nm with a reference wavelength at 650 nm with an M-UVmax microplate reader.{{Ishiyama M, Miyazono Y, Sasamoto K, Ohkura Y, Ueno K. A highly water-soluble disulfonated tetrazolium salt as a chromogenic indica
产品功能
1-methoxyPMS is a photochemically stable electron mediator between NAD(P)H and tetrazolium dyes. It is combined with WST-8 (CSN12409) in cell vitality assay (cck-8 assay).
参考文献
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