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CAS No.: 128-53-0

N-乙基马来酰亚胺 Catalog No. CSN23531

Synonyms: N-乙基马来酰亚胺;NEM

N-Ethylmaleimide is an irreversible inhibitor of all cysteine peptidases, it's an organic compound derived from maleic acid.

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技术信息

生物活性

描述N-Ethylmaleimide (NEM), a reagent that alkylates free sulfhydryl groups, is a cysteine protease inhibitor^[2]. N-ethylmaleimide (NEM) was shown to be a highly effective inhibitor of the following coupled mitochondrial processes: oxidative phosphorylation, ATP-32Pi exchange, Pi-induced light scattering and configurational changes, State III respiration, valinomycin-induced translocation of potassium with Pi as the anion, and calcium accumulation in presence of Pi. Also NEM, even et levels 2-3 times greater than those required to inhibit oxidative phosphorylation in intact mitochondria, did not inhibit coupled processes in submitochondrial particles^[3]. When D. salina thylakoid membranes are treated with N-ethylmaleimide, both photophosphorylation and the inducible CF1 ATPase activity are partially (approx. 60%) inhibited. Treatment of the purified, latent, D. salina CF1 with low concentrations of N-ethylmaleimide also results in the partial (approx. 60%) inhibition of the inducible ATPase activity (IC50 approximately 50 mM). N-ethylmaleimide-induced inhibition of the ATPase activity of either membrane-bound or solubilized CF1 is partially reversed by either prolonged incubation at low concentrations of N-ethylmaleimide or short incubation times at high concentrations of N-ethylmaleimide^[4].
参考文献

[2] Wu KH, Tai PC. Cys32 and His105 are the critical residues for the calcium-dependent cysteine proteolytic activity of CvaB, an ATP-binding cassette transporter. J Biol Chem. 2004 Jan 9;279(2):901-9
[3] Specific inhibition of phosphate transport in mitochondria by N-ethylmaleimide
[4] Selman-Reimer S, Duhe RJ, Selman BR. N-Ethylmaleimide inhibition of the catalytic activities of the Dunaliella salina coupling factor 1 (CF1) and the restoration of the inhibition of the CF1 ATPase activity by N-ethylmaleimide. Biochim Biophys Acta. 1985 Dec 16;810(3):325-31

实验方案

计算器摩尔计算器总药量计算器工作液计算器
储存液制备
- 1 mg
- 5 mg
- 10 mg
- 1 mM
- 7.9917 mL
- 39.9584 mL
- 79.9169 mL
- 5 mM
- 1.5983 mL
- 7.9917 mL
- 15.9834 mL
- 10 mM
- 0.7992 mL
- 3.9958 mL
- 7.9917 mL

技术信息

CAS号

128-53-0

储存条件

粉末: Keep in dark place,Inert atmosphere,2-8°C

液体: -20°C:3-6 月 -80°C:12月

分子式

C6H7NO2

运输

蓝冰

分子量

125.13

别名

N-乙基马来酰亚胺;NEM

溶解度

DMSO	23.0 mg/mL (183.8 mM)
Water	23.0 mg/mL (183.8 mM)
Ethanol	24.0 mg/mL (191.8 mM)

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N-乙基马来酰亚胺 Catalog No. CSN23531

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