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CAS No.: 4311-88-0
Synonyms: Nec-1
Necrostatin-1 is an inhibitor of RIP1 and can decrease TNF-α-induced necroptosis with EC50 of 490 nM.
生物活性
靶点 | RIP Kinase 1 EC50:490nM |
实验方案
技术信息
CAS号 | 4311-88-0 | 储存条件 |
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分子式 | C13H13N3OS | 运输 | 蓝冰 | |||||||||||
分子量 | 259.33 | 别名 | Nec-1 | |||||||||||
溶解度 |
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动物实验配方 |
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Cell Lines | Concentration | Assay Type | Time | Activity Description | Data Sources |
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8505c | 100 μM | Cell Viability Assay | 24 h | increases cellular survival | 22136818 |
A549 | 50 μM | Cell Viability Assay | 24 h | inhibits MMS-induced cell death | 26472723 |
AGS | 60 μm | Cell Viability Assay | 1 h | prevents shikonin-induced cell death | 24463199 |
BMDM | 10 μM | Function Assay | 30 min | protects cells from TAKI-induced LDH release | 26381601 |
BxPC-3 | 20 μM | Function Assay | 24 h | decreases the early necrotic cells | 26000607 |
C6 | 1 mmol/L | Cell Viability Assay | 3 h | attenuates Shikonin induced glioma cell death | 23840441 |
C6 | 1 mmol/L | Cytotoxicity Assay | 3 h | blocks shikonin induced necrosis | 23840441 |
C6 | 1 mmol/L | Function Assay | 1.5-3 h | suppresses the expression of RIP-1 caused by shikonin | 23840441 |
HL60 | 60 μM | Apoptosis Assay | 12 h | enhances shikonin-induced apoptosis | 22837689 |
HL60 | 60 μM | Function Assay | 12 h | augments the caspase-3 activity | 22837689 |
HL60 | 60 μM | Function Assay | 12 h | increases the activity of caspases, caspase 8 and 9 | 22837689 |
HL60/Adr | 60 μM | Apoptosis Assay | 12 h | enhances shikonin-induced apoptosis | 22837689 |
HL60/Adr | 60 μM | Function Assay | 12 h | augments the caspase-3 activity | 22837689 |
HL60/Adr | 60 μM | Function Assay | 12 h | increases the activity of caspases, caspase 8 and 9 | 22837689 |
HT-22 | 10 μM | Cell Viability Assay | 12 h | protects against glutamate-induced cell death | 23307752 |
HT-22 | 25 μM | Function Assay | 0–30 min | inhibits ERK Activation induced by glutamate | 23307752 |
HT-22 | 10 μM | Cytotoxicity Assay | 12 h | protects against cell death induced by 5 mmol/L glutamate | 17760869 |
Huh7 | 50 µM | Cell Viability Assay | 24/48 h | prevents cell death of rAdHCV co-infected Huh7 cells | 24973240 |
Jurkat | 50/ 100/200 μm | Cytotoxicity Assay | 1/3 h | reduces Naegleria fowleri-induced cytotoxicity | 21535020 |
Jurkat | 200 μm | Function Assay | 30 min | reduces Naegleria fowleri-induced reactive oxygen species (ROS) generation | 21535020 |
K562 | 60 μM | Apoptosis Assay | 12 h | enhances shikonin-induced apoptosis | 22837689 |
K562 | 60 μM | Function Assay | 12 h | augments the caspase-3 activity | 22837689 |
K562 | 60 μM | Function Assay | 12 h | increases the activity of caspases, caspase 8 and 9 | 22837689 |
K562/Adr | 60 μM | Apoptosis Assay | 12 h | enhances shikonin-induced apoptosis | 22837689 |
K562/Adr | 60 μM | Function Assay | 12 h | augments the caspase-3 activity | 22837689 |
K562/Adr | 60 μM | Function Assay | 12 h | increases the activity of caspases, caspase 8 and 9 | 22837689 |
KMS-12-BM | 90 µM | Cytotoxicity Assay | 1 h | blocks BAY 11-7082 induced rapid cell swelling | 23527154 |
KMS-12-PE | 60 μM | Cell Viability Assay | 5 h | inhibits SHK-induced cell death | 25530098 |
L-540 | 60 μm | Function Assay | 1 h | prevents the generation of ROS | 24561519 |
L-540 | 60 μm | Function Assay | 1 h | prevents the mitochondrial membrane depolarization | 24561519 |
L-540 | 60 μm | Cell Viability Assay | 1 h | reduces the Givinostat/Sorafenib-induced cell death | 24561519 |
L929 | 50 μM | Function Assay | 12 h | inhibits TNFα-induced Bid cleavage | 25398540 |
L929 | 30 μM | Cell Viability Assay | 1 h | inhibits TNF-α-induced loss of cell viability | 25095742 |
L929 | 30 μM | Cell Viability Assay | 1 h | inhibits TNF-α-induced cleavage of Topo I | 25095742 |
L929 | 2/5 μg/ml | Function Assay | 24 h | reversed the autophagy induced by TNFα alone as well as TNFα + zVAD | 23941769 |
L929 | 2/5 μg/ml | Growth Inhibition Assay | 24 h | reverses the cell growth inhibition and cell death induced by TNFα alone as well as TNFα + zVAD | 23941769 |
L929 | 2 μg/ml | Function Assay | 24 h | promots caspase-6 (p20) activity and procaspase-6 cleavage | 23941769 |
L929 | 5 μg/ml | Function Assay | 24 h | blocks zVAD induced necroptosis and autophagy | 23941769 |
L929-A | 50 μM | Cell Viability Assay | 24 h | blocks TNFα-induced cell death | 25398540 |
L929-A | 50 μM | Function Assay | 12 h | blocks the cleavage of Caspase-3 and PARP | 25398540 |
L929-A | 50 μM | Function Assay | 12 h | inhibits the TNFα-induced loss of mitochondrial membrane permeability | 25398540 |
L929-N | 50 μM | Cell Viability Assay | 24 h | blocks TNFα-induced cell death | 25398540 |
L929-N | 50 μM | Function Assay | 12 h | blocks the cleavage of Caspase-3 and PARP | 25398540 |
L929sA | 10 μM | Apoptosis Assay | 1 h | inhibits the apoptotic response to TNF | 22362767 |
L929sA | 10 μM | Apoptosis Assay | 1 h | rescues cells expressing RIPK1ΔID from TNF-induced apoptosis | 22362767 |
L929sA | 10 μM | Apoptosis Assay | 1 h | abrogates the interaction of caspase-8 with FADD | 22362767 |
MEFs | 10 μM | Cell Viability Assay | 48 h | inhibits zVAD-promoted death of CNOT3-depleted MEFs | 26437789 |
MEFs | 2/6/20 μM | Cytotoxicity Assay | 18 h | inhibits TNFα-induced cell death in RelA KO MEFs | 23727581 |
MEFs | 20 μM | Function Assay | 1/2/4 h | suppresses TNFα-induced RIPK1 phosphorylation | 23727581 |
MiaPaCa-2 | 20 μM | Function Assay | 24 h | decreases the early necrotic cells | 26000607 |
MM.1S | 90 µM | Cytotoxicity Assay | 1 h | blocks BAY 11-7082 induced rapid cell swelling | 23527154 |
NCI-H28 | 10 μM | Cell Viability Assay | 24 h | prevents DAPE-induced reduction of NCI-H28 cell viability | 26004138 |
NIH3T3 | 10/50 μM | Function Assay | 1/3 h | ameliorates TNFα-driven complex formation | 23261677 |
NRK-52E | 20 μM | Cell Viability Assay | 24 h | protects cells from cell death caused by ischemia injury | 24351845 |
NRK-52E | 20 μM | Cell Viability Assay | 24 h | increases cell viability after TNF-α Stimulation and ATP Depletion | 24351845 |
NRK-52E | 20 μM | Cell Viability Assay | 24 h | inhibits increased Drp1 protein expression after TNF-α Stimulation and ATP Depletion | 24351845 |
OHC | 300 μM | Function Assay | - | increases noise-induced condensed nuclei | 24874734 |
OHC | 300 μM | Function Assay | - | decreases noise-induced swollen nuclei | 24874734 |
OHC | 300 μM | Function Assay | - | results in a reduction of noise-induced RIP1 and RIP3 immunofluorescence | 24874734 |
OHC | 300 μM | Function Assay | - | diminishes noise-induced AMPK activation | 24874734 |
OHC | 300 μM | Function Assay | - | increases the number of apoptotic OHCs without altering the levels of CC8 after noise exposure | 24874734 |
RMS13 | 40 μg/ml | Cell Viability Assay | 24 h | rescues GX15-070-induced loss of cell viability | 23744296 |
SGC-7901 | 30 μM | Cell Viability Assay | 1 h | suppresses oxaliplatin-mediated cell death | 25767076 |
SH-EP | 10 μM | Apoptosis Assay | 72 h | inhibits IAP inhibitor- and Lexatumumab-induced apoptosis | 22890322 |
SK-Hep1 | 60 μM | Function Assay | 18 h | blocks β-lapachone-induced morphological change, cell death and PI uptake | 24832602 |
SK-Hep1 | 60 μM | Function Assay | 18 h | inhibits β-Lapachone-induced leakage of HMGB-1 | 24832602 |
SK-Hep1 | 60 μM | Function Assay | 18 h | blocks β-lapachone-mediated PAR accumulation and AIF translocation to the cytosol | 24832602 |
SW13 | 100 μM | Cell Viability Assay | 24 h | increases cellular survival | 22136818 |
TE671 | 40 μg/ml | Cell Viability Assay | 24 h | rescues GX15-070-induced loss of cell viability | 23744296 |
TPC-1 | 100 μM | Cell Viability Assay | 24 h | increases cellular survival | 22136818 |
U87 | 1 mmol/L | Cell Viability Assay | 3 h | attenuates Shikonin induced glioma cell death | 23840441 |
U87 | 1 mmol/L | Cytotoxicity Assay | 3 h | blocks shikonin induced necrosis | 23840441 |
U87 | 1 mmol/L | Function Assay | 1.5-3 h | suppresses the expression of RIP-1 caused by shikonin | 23840441 |
ΔN-Karpas 299 | 20 μM | Cytotoxicity Assay | 16 h | inhibits CD30-induced cell death | 23545938 |
靶点 | Description | IC50 |
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RIP Kinase 1 | EC50:490nM |
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