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CAS No.: 1370046-40-4

SSE15206 Catalog No. CSN26117

Synonyms: SSE15206

SSE15206 is a microtubule depolymerizing agent which inhibits microtubule polymerization both in biochemical and cellular assays by binding to colchicine site in tubulin. It causes aberrant mitosis resulting in G2/M arrest due to incomplete spindle formation.

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400-920-2911

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生物活性

实验方案

技术信息

生物活性

描述Microtubules are highly dynamic structures that form spindle fibres during mitosis and are one of the most validated cancer targets. SSE15206 is a microtubule polymerization inhibitor with GI₅₀ value of 197 nM in HCT116 cells. SSE15206 also exhibited potent antiproliferative activities against several other cancer cell lines of different origins (leukaemia, breast, lung, and cervical) with sub-micromolar GI₅₀ values. Further, dose-dependent increase in phosphorylation of both histone H3 and MPM2 was observed in CT116 cells following treatment with SSE15206 (0.5 μM, 1 μM and 2 μM) for 4 and 8 hours indicating mitotic arrest. Similarly, treatment with SSE15206 for 8 and 24 hours increased mitotic index (percentage mitotic cells) compared to the DMSO control. Within four hours of treatment with 1 μM SSE15206, CT116 cells started accumulating in the G2/M phase of the cell cycle compared to the DMSO control. The G2/M arrest increased in a time-dependent manner and peaked at 12 hours. Moreover, treatment of three different cell types, HCT116, A549 and CAL-51 with SSE15206 (0.5 μM, 1 μM and 2 μM) for 24 hours, resulted in increased expression of p53 and p21 compared to DMSO control^[1].
作用机制SSE15206 inhibits microtubule polymerization by binding to colchicine site in tubulin^[1].
参考文献

[1] Manzoor S, Bilal A, Khan S, et al. Identification and characterization of SSE15206, a microtubule depolymerizing agent that overcomes multidrug resistance. Sci Rep. 2018;8(1):3305

实验方案

计算器摩尔计算器总药量计算器工作液计算器
储存液制备
- 1 mg
- 5 mg
- 10 mg
- 1 mM
- 2.6921 mL
- 13.4606 mL
- 26.9213 mL
- 5 mM
- 0.5384 mL
- 2.6921 mL
- 5.3843 mL
- 10 mM
- 0.2692 mL
- 1.3461 mL
- 2.6921 mL

技术信息

CAS号

1370046-40-4

储存条件

粉末: Sealed in dry,2-8°C

液体: -20°C:3-6 月 -80°C:12月

分子式

C19H21N3O3S

运输

蓝冰

分子量

371.45

别名

SSE15206

溶解度

DMSO	74.0 mg/mL (199.2 mM)
Water	Insoluble
Ethanol	Insoluble

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