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CAS No.: 246256-50-8
5(6)-TAMRA SE, also called 5(6)-TAMRA NHS, is consist of two parts, the fluorophore 5(6)-TAMRA and the reactive group N-Hydroxysuccinimide (NHS).
规格 | 价格 | 库存 |
---|---|---|
1mg | RMB 316 | 产品咨询 |
5mg | RMB 531 | 产品咨询 |
10mg | RMB 675 | 产品咨询 |
50mg | RMB 1688 | 产品咨询 |
100mg | RMB 2700 | 产品咨询 |
描述
5(6)-TAMRA SE, also called 5(6)-TAMRA NHS, is consist of two parts, the fluorophore 5(6)-TAMRA and the reactive group N-Hydroxysuccinimide (NHS).
保存条件
Store at -20°C in dark
使用方法
Guidance for conjugation of Fluorophore-NHS with protein: Step 1 Solutions & buffers 1 M Sodium Phosphate Buffer, pH 7.0 10x NaH2PO4 1 M 125 ml Na2HPO4 1 M 875 ml Reaction buffer Sodium Phosphate buffer (pH 7.0) 0.1 M 100 ml KOAc(pH 7.4) 75 mM 37.5 ml DTT 2 mM 2 ml Step 2 Make a 20 mM stock of Fluorophore-NHS (e.g. 5(6)-TAMRA NHS) in DMSO Step 3 Enzyme storage buffer HEPES–KOH (pH 7.4) 20 mM 20 ml KOAc (pH 7.4) 100 mM 50 ml DTT 2 mM 2 ml Glycerol 10% 100 ml Preparation: Pure lyophilized protein can be dissolved in reaction buffer to a final concentration of >2.0 mg ml-1. If the protein is already in solution, it should be brought into reaction buffer by dialyzing overnight at 4°C against 2 l reaction buffer, by gel filtration, or by repeated buffer exchange through a protein concentrator of desired
产品功能
5(6)-TAMRA SE, also called 5(6)-TAMRA NHS, is consist of two parts, the fluorophore 5(6)-TAMRA and the reactive group N-Hydroxysuccinimide (NHS). When proteins conjugated with 5(6)-TAMRA NHS through the reaction of NHS with the amine groups, the proteins are labeled with 5(6)-TAMRA with excitation wavelength of 540 nm and emission wavelength of 565 nm (MeOH).
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