Dansyl chloride is a sulfonyl chloride compound.

Store at -20°C in dark.

Protocol for dansyl chloride staining of polyacrylamide gels The staining suspension is prepared by dissolving 30 mg of dansyl chloride in a minimal volume of acetone and then making up the volume to 150 ml with 0.1 M carbonate-bicarbonate buffer, pH 9.2. 1. The gels after the electrophoresis run are soaked in 150 ml of 50% aqueous methanol with constant shaking with three changes at 10 min intervals. 2. Gels are removed from the 50% aqueous methanol and are stained with 150 ml of the staining suspension with constant shaking for 30 min. 3. A clear background for the gel is achieved by destaining the gel with 150 ml of the 50% aqueous methanol with three changes at 10 min intervals. 4. The protein bands on the gels stained with dansyl chloride are detected using a UV lamp and are photographed under a UV transilluminator (Fotodyne). {{Kumar TK, Raman B, Rao CM. Fluorescent staining for proteins on polyacrylamide gels with 5-dimethylamino-1-naphthalenesulfonyl chloride (dansyl chlorid

Dansyl chloride is a sulfonyl chloride compound. It is used to end tag amino groups of peptides and proteins; for derivatization of free amino acids and amines in neural samples and biological fluids; to study fluorescent conjugates of albumin, protein conformational changes studies and active site characterization.