DFHBI is a small molecular, fluorescence of which can be switched on when it bind to Broccoli RNA aptamer.

Store in dark at -20 ℃.

The protocol for use of DFHBI-1T in gel staining, or RNA imaging with dimeric Broccoli in live bacterial and mammalian cells can be found in the article{{Filonov GS, Jaffrey SR. RNA Imaging with Dimeric Broccoli in Live Bacterial and Mammalian Cells. Curr Protoc Chem Biol. 2016 Mar 16;8(1):1-28. doi: 10.1002/9780470559277.ch150174. PMID: 26995352; PMCID: PMC4829638.|https://pubmed.ncbi.nlm.nih.gov/26995352/}}.

DFHBI is a small molecular, fluorescence of which can be switched on when it bind to Broccoli RNA aptamer. DFHBI 1T possesses higher photostability than DFHBI, and can mimic GFP when activated by specific RNA sequence (DFHBI-1T aptamer) such as Spinach2 or Broccoli. Through validating the fluorescence of dimeric Broccoli−labeled transcripts in vitro and in cells upon DFHBI 1T, localization of RNA in live cells can be monitored.

[1] Filonov GS, Jaffrey SR. RNA Imaging with Dimeric Broccoli in Live Bacterial and Mammalian Cells. Curr Protoc Chem Biol. 2016 Mar 16;8(1):1-28. doi: 10.1002/9780470559277.ch150174. PMID: 26995352; PMCID: PMC4829638.