Fluorescein sodium salt has been used as a solute to study microvessel permeability.

Store in dark at room temperature.

Fluorescein sodium salt/Evans Blue uptake study Ringer solution: 117 mM NaCl, 4.7 mM KCl, 0.8 mM MgSO4, 24.8 mM NaHCO3, 1.2 mM KH2PO4, 2.5 mM CaCl2, 10 mM d-glucose, and 18 g/l bovine serum albumin. All perfusion solutions are maintained at 37 °C with a water bath. 1. Rats are anesthetized. Common carotid arteries are exposed and cannulated, and the jugular veins sectioned. 2. Perfusion with Ringer solution for 3 min, via peristaltic pump. 3. Perfusion with Ringer solution +1 g/l each of Fluorescein sodium salt (NF) and Evans Blue (EB) (mixed the night before to allow maximal binding of Evans Blue to albumin) for 1-3 min. 4. Washout with Ringer solution without dyes for 5 min. 5. Animals are decapitated and the brains are immediately removed, cerebral hemispheres are dissected away from the brainstem and cerebellum, and meninges and choroid plexuses removed. Hemispheres are mechanically homogenized in 3 ml 7.5% (w/v) trichloroacetic acid (TCA), and the resulting suspension divided in

Fluorescein sodium salt has been used as a solute to study microvessel permeability; as a test molecule to study blood barrier penetration; as a fluorescent tracer to study blood-brain barrier (BBB) and blood-spinal cord barrier (BSCB) permeability in rodent models; as a probe substrate in the study of organic anion transporting polypeptide (OATP) mediated drug transport in hepatocytes. It was also used to monitor in situ solute transportation into bone through fluorescence recovery after photobleaching (FRAP). λex 460 nm; λem 515 nm(lit.).