生物活性

• 描述Epigallocatechin-3-gallate (EGCG), also termed Epigallocatechol Gallate, the most abundant and most biologically active catechin from green tea, is a potential therapeutic agent for preventing neurodegenerative, inflammatory diseases and cancer. In vitro EGCG supplementation dose-dependently reduced neutrophil migration induced by chemokine interleukin (IL)-8 with an IC₅₀ lower than 1 mM and by cytokine-induced neutrophil chemoattractant-1 at a dose of 15 mg/ml or higher^[3]. PC12 cells cultured without serum were administrated with EGCG (0.1 and 1 μM) for 24h which resulted in improved cell morphology and promoted neurite outgrowth, and significant lower cell mortality as compared with serum free group (~40%). There was a gradual increase in neurite length after exposure to EGCG (0.1 and 1 μM); at 4 days of EGCG (1 μM) treatment 40 ± 0.9 and 25.2 ± 2.2% of detected cells exhibited extended neurites, respectively^[4]. EGCG decreased cell viability as concentration- and time-dependently, with IC₅₀ values of 14.17 µM for human breast cancer cell line T47D and 193.10 µM for normal human foreskin fibroblast cell line HFF cells, through down-regulation of PI3K/AKT and telomerase^[5].
• 细胞研究
• Cell Data

  Cell Lines	Concentration	Assay Type	Time	Activity Description	Data Sources
  CHO cells	-	Cytotoxic assay	48 h	Cytotoxicity against CHO cells expressing OATP1B3 haplotype 1 after 48 hrs by fluorescence based CellTiter-Glo assay, IC50=3.2 μM	23327877
  HSC-T6 cells	-	Function assay	48 h	Antifibrotic activity against rat HSC-T6 cells assessed as inhibition of proliferation after 48 hrs by BrdU incorporation assay, IC50=9.9 μM	21504848
  HSC-T6 cells	-	Proliferation assay	-	Antiproliferative activity against rat HSC-T6 cells assessed as reduction in cell viability, IC50=29.8 μM	25322455

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• 临床研究

• NCT号	适应症或疾病	临床期	招募状态	预计完成时间	地点
  NCT01360320	Colorectal Serrated Adenomas ... more >> Colorectal Tubular Adenomas Colorectal Villous Adenomas Colorectal Tubulovillous Adenomas Collapse <<	Phase 2	Unknown	March 2018	Germany ... more >> Ostalb-Klinikum Aalen, Medizinische Klinik 1, Sekretariat Prof. Kleber Recruiting Aalen, Germany, 73430 Contact: Caroline Walny       caroline.walny@ostalb-klinikum.de    Principal Investigator: Gerhard Kleber, MD          Klinikum Altenburger Land, Gastroenterologie Not yet recruiting Altenburg, Germany, 04600 Contact: Michael Repp, MD       michael.repp@klinikum-altenburgerland.de    Principal Investigator: Michael Repp, MD          Klinikum Augsburg, III. Med. Klinik Recruiting Augsburg, Germany, 86156 Contact: Tanja Hilmer       Tanja.Hilmer@klinikum-augsburg.de    Krankenhaus Bietigheim-Bissingen, Klinik für Innere Medizin, Gastroenterologie, Hämato-Onkologie Recruiting Bietigheim-Bissingen, Germany, 74321 Contact: Ulla Hegmann       hegmul01@kliniken-lb.de    Principal Investigator: Siegfried Walker, MD          Krankenhaus Buchholz, Abteilung Innere Medizin Recruiting Buchholz, Germany, 21244 Contact: Dirk Fahrenholz, MD       Dirk.Fahrenholz@krankenhaus-buchholz.de    Principal Investigator: Dirk Fahrenholz, MD          Kliniken der Stadt Köln gGmbH, Krankenhaus Holweide -Medizinische Klinik- Recruiting Cologne, Germany Contact: Claudia Kaiser-Stolz       Kaiser-Stolz@kliniken-koeln.de    Principal Investigator: Ulrich Huegle, MD          Klinikum Esslingen, Klinik für Innere Medizin, Onkologie, Gastroenterologie Recruiting Esslingen, Germany, 73730 Contact: Stephanie Klink       s.klink@kliniken-es.de    Principal Investigator: Michael Geissler, MD          Universitätsklinikum der Ernst-Moritz-Arndt-Universität Greifswald, Klinik und Poliklinik für Innere Medizin A Recruiting Greifswald, Germany, 17475 Contact: Eckhard Weber       eckhard.weber@uni-greifswald.de    Principal Investigator: Julia Mayerle, MD          Dr. Zeisler, Praxis für Innere Medizin und Gastroenterologie Recruiting Halle, Germany, 06108 Contact: Brigitte Schneider       gastrozeisler@yahoo.de    Principal Investigator: Thomas Zeisler, MD          Dres. Fechner/Behrens/Steudel - Gastroenterologisch-Onkologische Praxisklinik Recruiting Halle, Germany, 06108 Contact: Anja Schlenk       bsf@gastro-halle.de    Principal Investigator: Ruediger Behrens, MD          Dr. Frank-Gleich Praxis für Innere Medizin und Gastroenterologie Recruiting Halle, Germany, 06110 Contact: Ute Kabisch       u.kabisch@onkomedic.de    Principal Investigator: Stefanie Frank-Gleich, MD          Universitätsklinikum Halle, Klinik für Innere Medizin I Recruiting Halle, Germany, 06120 Contact: Teuber Evelin       evelin.teuber@medizin.uni-halle.de    Principal Investigator: Thomas Seufferlein, MD          Sub-Investigator: Thomas J. Ettrich, MD          Klinikum Ludwigsburg, Medizinische Klinik I Recruiting Ludwigsburg, Germany, 71640 Contact: Samuel Raad       samuel.raad@kliniken-lb.de    Principal Investigator: Karel Caca, MD          Diakoniekrankenhaus Mannheim, Medizinische Klinik II Recruiting Mannheim, Germany, 68163 Contact: Ulrich Damian, MD       u.damian@diako-ma.de    Principal Investigator: Ulrich Damian, MD          II. Medizinische Klinik und Poliklinik der TU München, Klinikum rechts der Isar Recruiting Munich, Germany, 81675 Contact: Jens Zimmermann       Studiensekretariat.med2@lrz.tum.de    Principal Investigator: Jens T. Siveke, MD          Klinik Mühldorf Abt.Gastroenterologie Recruiting Mühldorf am Inn, Germany, 84453 Contact: Melanie Mühlberger       melanie.muehlberger@kliniken-muehldorf.de    Principal Investigator: Gerhard Fuechsl, MD          Klinikum Bogenhausen, Interdisziplinäre Onkologische Tagklinik Recruiting München, Germany, 81525 Contact: Romy Petermann       romy.petermann@klinikum-muenchen.de    Principal Investigator: Martin Fuchs, MD          Regio Kliniken Pinneberg Not yet recruiting Pinneberg, Germany, 25421 Contact: Stefan Tardos, MD       stefan.tardos@regiokliniken.de    Principal Investigator: Stefan Tardos, MD          Klinikum St. Elisabeth, I. Medizinische Klinik Recruiting Straubing, Germany, 94315 Contact: Stefanie Wolf       stefanie.wolf@klinikum-straubing.de    Principal Investigator: Weigert Norbert, MD          Universitätsklinikum Ulm, Klinik für Innere Medizin I Recruiting Ulm, Germany, 89081 Contact: Yvonne Kriebisch       yvonne.kriebisch@uniklinik-ulm.de    Principal Investigator: Goetz von Wichert, MD          Evangelisches Krankenhaus Wesel, Abteilung Innere Medizin Recruiting Wesel, Germany, 46485 Contact: Adelheid Rosendahl       rosendahl@evkwesel.de    Principal Investigator: Dirk Hartnack, MD Collapse <<
  NCT00917735	Breast Cancer	Phase 2	Completed	-	United States, Minnesota ... more >> Fairview Southdale Breast Center Edina, Minnesota, United States, 55435 Fairview Maple Grove Breast Center Maple Grove, Minnesota, United States, 55369 University of Minnesota Medical Center (UMMC) Breast Clinic Minneapolis, Minnesota, United States, 55455 Park Nicollet Institute St. Louis Park, Minnesota, United States, 55426 Food Science and Nutrition, University of Minnesota St. Paul, Minnesota, United States, 55108 Collapse <<
  NCT00253643	Precancerous Condition ... more >> Prostate Cancer Collapse <<	Not Applicable	Completed	-	United States, Oregon ... more >> Kaiser Permanente Center for Health Research Portland, Oregon, United States, 97227 OHSU Knight Cancer Institute Portland, Oregon, United States, 97239-3098 Veterans Affairs Medical Center - Portland Portland, Oregon, United States, 97239 Collapse <<

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• 参考文献

• [1] Pae M, Wu D. Immunomodulating effects of epigallocatechin-3-gallate from green tea: mechanisms and applications. Food Funct. 2013 Sep;4(9):1287-303.

  [2] Khan N, Afaq F, et al. Targeting multiple signaling pathways by green tea polyphenol (-)-epigallocatechin-3-gallate. Cancer Res. 2006 Mar 1;66(5):2500-5.

  [3] Donà M, Dell'Aica I, Calabrese F, Benelli R, Morini M, Albini A, Garbisa S. Neutrophil restraint by green tea: inhibition of inflammation, associated angiogenesis, and pulmonary fibrosis. J Immunol. 2003 Apr 15;170(8):4335-41. doi: 10.4049/jimmunol.170.8.4335. PMID: 12682270.

  [4] Reznichenko L, Amit T, Youdim MB, Mandel S. Green tea polyphenol (-)-epigallocatechin-3-gallate induces neurorescue of long-term serum-deprived PC12 cells and promotes neurite outgrowth. J Neurochem. 2005 Jun;93(5):1157-67. doi: 10.1111/j.1471-4159.2005.03085.x. PMID: 15934936.

  [5] Moradzadeh M, Hosseini A, Erfanian S, Rezaei H. Epigallocatechin-3-gallate promotes apoptosis in human breast cancer T47D cells through down-regulation of PI3K/AKT and Telomerase. Pharmacol Rep. 2017 Oct;69(5):924-928. doi: 10.1016/j.pharep.2017.04.008. Epub 2017 Apr 13. PMID: 28646740.

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