|1-(3-乙基-5-甲氧基苯并[d]噻唑-2(3H)-亚基)丙-2-酮|CsnPharm

CAS No.: 300801-52-9

(E/Z)-TG003 Catalog No. CSN22563

Synonyms: (E/Z)-TG003

TG003 is a potent, reversible ATP-competitive inhibitor of Cdc-2 like kinase (Clk) with IC50s of 20 nM and 15 nM for Clk1/Sty and Clk4.

规格	价格	促销价格	库存	数量

400-920-2911

sales@csnpharm.cn

tech@csnpharm.cn

加入购物车大规格询价

加入收藏

产品咨询

400-920-2911

sales@csnpharm.cn

tech@csnpharm.cn

纯度 & 质量文件批次:

靶点选择性

生物活性

实验方案

技术信息

生物活性

描述The Clk (Cdc2-like kinase) family, consisting of at least four members, has been implicated in regulating alternative splicing by phosphorylation of SR (serine/arginine-rich) proteins. TG003 is a potent and ATP-competitive inhibitor of Clk with IC₅₀s of 15, 20, 200 nM for mClK4, mClK1 and mClK2, respectively. TG003 (1 μM; 4 h) inhibited SF2/ASF-dependent splicing in HeLa cytosolic S100 extract by suppression of Clk1/Sty-mediated phosphorylation. It attenuated the splicing of β-globin pre-mRNA in S100 extract complemented with rSF2/ASF. When treated with 10 μM TG003 for 12 hours, HA-Clk1/Sty was localized in nuclear speckles in HA-Clk1/Sty-overexpressing HeLa cells with suppressed phosphorylation of SR proteins. In vivo, the abnormal development phenotype of the Xenopus embryos was rescued when they were incubated with 10 μM TG003^[2].
细胞研究

Cell Data

Cell Lines	Concentration	Assay Type	Time	Activity Description	Data Sources
human STO cells	-	Function assay	-	Modulation of Clk/sty pre-mRNA splicing in human STO cells assessed as concentration required to increase in mature Clk2 mRNA level by RT-PCR analysis, EC50=6.6 μM	25221649

参考文献

[1] Muraki M, Ohkawara B, Hosoya T, et al. Manipulation of alternative splicing by a newly developed inhibitor of Clks. J Biol Chem. 2004;279(23):24246-54.
[2] Muraki M, et al. Manipulation of alternative splicing by a newly developed inhibitor of Clks. J Biol Chem. 2004 Jun 4;279(23):24246-54

实验方案

计算器摩尔计算器总药量计算器工作液计算器
储存液制备
- 1 mg
- 5 mg
- 10 mg
- 1 mM
- 4.0108 mL
- 20.0538 mL
- 40.1077 mL
- 5 mM
- 0.8022 mL
- 4.0108 mL
- 8.0215 mL
- 10 mM
- 0.4011 mL
- 2.0054 mL
- 4.0108 mL

技术信息

CAS号

300801-52-9

储存条件

粉末: Keep in dark place,Sealed in dry,2-8°C

液体: -20°C:3-6 月 -80°C:12月

分子式

C13H15NO2S

运输

蓝冰

分子量

249.33

别名

(E/Z)-TG003

溶解度

DMSO	115.0 mg/mL (461.2 mM)
Water	insoluble

动物实验配方

大规格询价

号为必填项

姓名: 邮箱: 手机号:

留言:

提交

(E/Z)-TG003 Catalog No. CSN22563

靶点选择性

请登录您的专属账户

确认信息