|(8S,9S,10R,13S,14S,17S)-17-((R)-2-羟基-6-甲基庚烷-2-基)-10,13-二甲基-2,3,4,7,8,9,10,11,12,13,14,15,16,17-十四氢-1H-环戊二烯并[a]菲-3-醇|CsnPharm

CAS No.: 516-72-3

20(S)-Hydroxycholesterol Catalog No. CSN25227

Synonyms: 20α-Hydroxycholesterol;20(S)-hydroxy Cholesterol;20(S)-羟基胆固醇

20(S)-Hydroxycholesterol is an allosteric activator of the Hedgehog signaling pathway Smoothened (Smo) oncoprotein. It activates Hedgehog (Hh) signaling with EC50 value of ~ 3μM for induction of Hh reporter gene transcription in NIH 3T3 cells.

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400-920-2911

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技术信息

生物活性

描述20(S)-hydroxyCholesterol (20α-Hydroxycholesterol) is an allosteric activator of the oncoprotein smoothened (Smo) that activates the hedgehog (Hh) signaling pathway with an EC50 of 3 μM in a gene transcription reporter assay using NIH3T3 cells^[1]. Like human and mouse MSCs, 20S oxysterol expressed pro-osteogenic, pro-myogenic, and anti-adipogenic differentiation potential in cBMSCs. Moreover, 20(S)-Hydroxycholesterol induced markers of osteogenic genes and myogenic regulatory factors when exposed to cBMSCs treated with their specific medium^[2]. Treatment of M2-10B4 cells with the osteogenic oxysterol 20S completely inhibited adipocyte formation induced by troglitazone after 10 days. Furthermore, 20S and Shh greatly inhibited PPARgamma2 promoter activity induced by CCAAT/enhancer-binding protein alpha overexpression^[3].
参考文献

[1] Nedelcu D, Liu J, Xu Y, Jao C, Salic A. Oxysterol binding to the extracellular domain of Smoothened in Hedgehog signaling. Nat Chem Biol. 2013 Sep;9(9):557-64
[2] Adhikari R, Chen C, Kim WK. Effect of 20(S)-Hydroxycholesterol on Multilineage Differentiation of Mesenchymal Stem Cells Isolated from Compact Bones in Chicken. Genes (Basel). 2020 Nov 17;11(11):1360
[3] Kim WK, Meliton V, Amantea CM, Hahn TJ, Parhami F. 20(S)-hydroxycholesterol inhibits PPARgamma expression and adipogenic differentiation of bone marrow stromal cells through a hedgehog-dependent mechanism. J Bone Miner Res. 2007 Nov;22(11):1711-9

实验方案

计算器摩尔计算器总药量计算器工作液计算器
储存液制备
- 1 mg
- 5 mg
- 10 mg
- 1 mM
- 2.4835 mL
- 12.4176 mL
- 24.8353 mL
- 5 mM
- 0.4967 mL
- 2.4835 mL
- 4.9671 mL
- 10 mM
- 0.2484 mL
- 1.2418 mL
- 2.4835 mL

技术信息

CAS号	516-72-3	储存条件	粉末 Inert atmosphere,Room Temperature 液体 -20°C:3-6 月 -80°C:12月
分子式	C27H46O2	运输	蓝冰
分子量	402.65	别名	20α-Hydroxycholesterol;20(S)-hydroxy Cholesterol;20(S)-羟基胆固醇

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