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CAS No.: 25535-16-4

碘化丙啶 Catalog No. CSN64556

Synonyms: 碘化丙啶;PI

Propidium iodide (PI) is a red-fluorescent nuclear and chromosome counterstain which can bind to double stranded DNA by intercalating between base pairs.

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生物活性

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技术信息

生物活性

描述Propidium iodide (PI) is a red-fluorescent nuclear and chromosome counterstain which can bind to double stranded DNA by intercalating between base pairs. It is a cell impermeant dye, which can be excluded from viable cells. Thus it is widely used for the evaluation of apoptosis in different experimental models. Propidium iodide is excited at 488 nm and, with a relatively large Stokes shift, emits at a maximum wavelength of 617 nm.
General protocol:

1. Trypsinize and harvest cells in the appropriate manner and wash in PBS.

2. Fix cells into 0.5 ml 70% EtOH for at least 1h. 70% EtOH should be pre-cooled to -20 °C overnight.

PAUSE POINT: Cells can be stored in ethanol solution at –20°C for several weeks.

3. Spin down cells for 2 min at 2,000 rpm.

4. Discard the supernatant. Be careful to avoid cell loss.

5. Wash in PBS for two times as: resuspend cell pellet in 0.5mL 1xPBS, spin down cells for 2 min at 2,000 rpm, discard the supernatant and repeat these steps.

6. Resuspend cell pellet in 0.5 ml PBS containing 10 μg/ml RNase A and 20 μg/ml PI stock solution, transfer to FACS tubes.

Note: The addition of RNase A could ensure that only DNA is stained.

7. Incubate at room temperature in the dark for 30 min.

8. Analyze cells by flow cytometry. Use 488-nm laser line for excitation. Measure red fluorescence (>600 nm) and side scatter.

Optimal protocol:

Fluorochrome solution: 0.1% sodium citrate (wt/v), 0.1% Triton X-100

实验方案

计算器摩尔计算器总药量计算器工作液计算器
储存液制备
- 1 mg
- 5 mg
- 10 mg
- 1 mM
- 1.4961 mL
- 7.4806 mL
- 14.9612 mL
- 5 mM
- 0.2992 mL
- 1.4961 mL
- 2.9922 mL
- 10 mM
- 0.1496 mL
- 0.7481 mL
- 1.4961 mL

技术信息

CAS号	25535-16-4	储存条件	粉末 Keep in dark place,Sealed in dry,2-8°C 液体 -20°C:3-6 月 -80°C:12月
分子式	C27H34I2N4	运输	蓝冰
分子量	668.39	别名	碘化丙啶;PI

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