|4-氯-2-硝基-1-(苯基磺酰基)苯|CsnPharm

CAS No.: 86030-08-2

BTB-1 Catalog No. CSN20459

Synonyms: NSC 156750;NSC 658180

BTB1 an ATP-competitive and selective inhibitor of the mitotic motor protein Kif18A with IC50 value of 1.7 μM.

规格	价格	促销价格	库存	数量

400-920-2911

sales@csnpharm.cn

tech@csnpharm.cn

加入购物车大规格询价

加入收藏

产品咨询

400-920-2911

sales@csnpharm.cn

tech@csnpharm.cn

纯度 & 质量文件批次:

靶点选择性

生物活性

实验方案

技术信息

生物活性

靶点 KIF18A

IC50:1.7μM
描述Kif18A is a mitotic motor protein essential for the correct alignment of chromosomes at the spindle equator. BTB1 potently inhibits the ATPase activity of Kif18A with an IC50 value of 1.69μM. The re-synthesized BTB1 inhibited His-Kif18A^motor with equal potency (IC50=1.86μM). The flushing in of 100μM BTB1 almost completely inhibited His-Kif18A^FL-dependent microtubule motility. After the wash-out of BTB1, His-Kif18A^FL regained most of its activity. The ATPase activity of His-Kif18A^motor was not affected by BTB1(100μM) in the absence of microtubules. BTB1 significantly inhibited microtubule-stimulated ATPase activity of His-Kif18Amotor as compared to the DMSO-treated group. Incubation of HeLa cells with BTB1 (30 and 40μM) for 18h induced the accumulation of cells in mitosis^[3].
作用机制BTB1 is a potent and selective Kif18A inhibitor that reversibly inhibits the motility of Kif18A in an ATP-competitive manner^[3].
参考文献

[1] Braun J, Mockel MM, et al. Synthesis and biological evaluation of optimized inhibitors of the mitotic kinesin Kif18A. ACS Chem Biol. 2015 Feb 20;10(2):554-60.
[2] Catarinella M, Gruner T, et al. BTB-1: a small molecule inhibitor of the mitotic motor protein Kif18A. Angew Chem Int Ed Engl. 2009;48(48):9072-6.
[3] Catarinella M, Grüner T, Strittmatter T, Marx A, Mayer TU. BTB-1: a small molecule inhibitor of the mitotic motor protein Kif18A. Angew Chem Int Ed Engl. 2009;48(48):9072-9076. doi:10.1002/anie.200904510

实验方案

计算器摩尔计算器总药量计算器工作液计算器
储存液制备
- 1 mg
- 5 mg
- 10 mg
- 1 mM
- 3.3589 mL
- 16.7946 mL
- 33.5893 mL
- 5 mM
- 0.6718 mL
- 3.3589 mL
- 6.7179 mL
- 10 mM
- 0.3359 mL
- 1.6795 mL
- 3.3589 mL

技术信息

CAS号

86030-08-2

储存条件

粉末: Sealed in dry,Room Temperature

液体: -20°C:3-6 月 -80°C:12月

分子式

C12H8ClNO4S

运输

蓝冰

分子量

297.71

别名

NSC 156750;NSC 658180

溶解度

DMSO	60.0 mg/mL (201.5 mM)
Water	Insoluble
Ethanol	2.0 mg/mL (6.7 mM)

动物实验配方

大规格询价

号为必填项

姓名: 邮箱: 手机号:

留言:

提交

BTB-1 Catalog No. CSN20459

靶点选择性

请登录您的专属账户

确认信息