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CAS No.: 9003-98-9

脱氧核糖核酸酶 Catalog No. CSN96537

Synonyms: DNase (RNase free

DNase I is an endonuclease that acts on phosphodiester bonds adjacent to pyrimidines to produce polynucleotides with terminal 5′-phosphates.

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纯度 & 质量文件批次:

靶点选择性

生物活性

描述DNase I is an endonuclease that acts on phosphodiester bonds adjacent to pyrimidines to produce polynucleotides with terminal 5′-phosphates. In the presence of Mg²⁺, DNAse I cleaves each strand of DNA independently and the cleavage sites are random. Both DNA strands are cleaved at approximately the same site in the presence of Mn²⁺^[1]. Divalent cations such as Mn²⁺, Ca²⁺, Co²⁺, and Zn²⁺ are activators of the enzyme. A concentration of 5 mM Ca²⁺ stabilizes the enzyme against proteolytic digestion. The pH optimum is found to be between 7 and 8^[1]. 2-Mercaptoethanol, chelators, sodium dodecyl sulfate (SDS) and actin are known to inhibit the enzyme activity.
One Kunitz unit will produce a change in A₂₆₀ of 0.001 per min per mL at pH 5.0 at 25 °C, using DNA, Type I or III, as substrate.
Preparation Note:
10 mg/mL solution of DNAse I in 0.15 M NaCl may lose <10% of its activity when stored for a week in aliquots at −20 °C.
The same solutions stored in aliquots at 2-8 °C can lose approximately 20% activity.
It remains active for up to five hours at 60 °C between pH 5 and 7, and loses activity in <10 minutes at 68 °C.
It loses activity at the rate of 6%/hour in acetate buffer (pH 5.0) and tris buffer ((pH 7.2) at 1 mg/mL concentration.
参考文献

[1] Sambrook, J. and Russell, D.W. (2001) Molecular Cloning: A Laboratory Manual. 3rd Edition, Vol. 1, Cold Spring Harbor Laboratory Press, New York.
[2] Enzymes of Molecular Biology

实验方案

计算器摩尔计算器总药量计算器工作液计算器

技术信息

CAS号	9003-98-9		储存条件	粉末 Keep in dark place,Sealed in dry,Store in freezer, under -20°C 液体 -20°C:3-6 月 -80°C:12月
分子式			运输	蓝冰
分子量	N/A		别名	DNase (RNase free

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