| Escherichia coli BL21 (DE3) cells | - | Function assay | 90 mins | Activity of N-terminus hexaHis-tagged human TNSK2 expressed in Escherichia coli BL21 (DE3) cells using biotinylated NAD+ as substrate after 90 mins by Western blot analysis, EC50=0.2 μM | 22233320 |
| HEK293 cells | - | Function assay | - | Displacement of IWR-PB from Axin2 in HEK293 cells by Western blot method | 19125156 |
| human DLD1 cells | - | Function assay | 2 h | Induction of Axin2 stabilization in human DLD1 cells assessed as decreased transcription of Axin2 after 2 hrs by RT-PCR method. | 19125156 |
| human HEK293T cells | - | Function assay | - | Inhibition of beta-casein-dependent canonical Wnt3 pathway in human HEK293T cells by luciferase reporter gene assay, IC50=0.026 μM | 22191557 |
| human HT29 cells | - | Function assay | 24 h | Inhibition of Wnt signaling in human HT29 cells assessed as inhibition of beta-catenin-mediated Tcf/Lef transcriptional activity after 24 hrs by dual luciferase reporter gene assay relative to control, IC50=24.4 μM | 24950489 |
| human HT29 cells | 25 μM | Function assay | 24 h | Inhibition of Wnt signaling in human HT29 cells assessed as inhibition of beta-catenin-mediated Tcf/Lef transcriptional activity at 25 uM after 24 hrs by dual luciferase reporter gene assay relative to control in presence of GSK-3beta inhibitor LiCl | 24950489 |
| human SW480 cells | - | Function assay | 40 to 48 h | Inhibition of tankyrase in human SW480 cells assessed as degradation of beta catenin after 40 to 48 hrs, IC50=0.25 μM | 23701517 |
| human SW480 cells | - | Function assay | 24 h | Inhibition of tankyrase in human SW480 cells assessed as accumulation of axin2 after 24 hrs by Hoechst dye-based method | 23701517 |
| mouse L-Wnt-STF cells | - | Function assay | - | Inhibition of TNKS-2 in mouse L-Wnt-STF cells assessed as disruption of Wnt signaling after 24 hrs by luciferase reporter gene assay, IC50=0.056 μM | 24527792 |
| mouse L-Wnt-STF cells | - | Function assay | - | Inhibition of TNKS-1 in mouse L-Wnt-STF cells assessed as disruption of Wnt signaling after 24 hrs by luciferase reporter gene assay, IC50=0.131 μM | 24527792 |
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