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CAS No.: 1003197-00-9

MitoSOX Red Catalog No. CSN89835

Synonyms: MitoHE;Mito-hydroethidine

MitoSOX Red is a novel fluorescent probe that can permeate the cell membrane and quickly and specifically bind to the mitochondria of living cells.

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400-920-2911

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tech@csnpharm.cn

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400-920-2911

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靶点选择性

生物活性

实验方案

技术信息

生物活性

描述MitoSOX Red is a novel fluorescent probe that can permeate the cell membrane and quickly and specifically bind to the mitochondria of living cells. A general protocol suggested as follow:
1. Preparation of MitoSOX Red working solution
1.1 Preparation of the stock solution
Dissolve 50 μg MitoSOX Red in 13 μL DMSO to obtain 5 mM of stock solution.
Note: It is recommended to store the stock solution at -20°C and -80°C away from light and avoid repetitive freeze-thaw cycles.
1.2 Preparation of MitoSOX Red working solution
Dilute the stock solution in serum-free cell culture medium or PBS to obtain 1-10 μM of working solution.
Note: Please adjust the concentration of MitoSOX Red working solution according to the actual situation.
2. Cell staining
2.1 Suspension cells（6-well plate）
a. Centrifuge at 1000 g at 4°C for 3-5 minutes and then discard the supernatant. Wash twice with PBS, 5 minutes each time.The cell density is 1×106/mL.
b. Add 1 mL of working solution, and then incubate at room temperature for 5-30 minutes.
c. Centrifuge at 400 g at 4°C for 3-4 minutes and then discard the supernatant.
d. Wash twice with PBS, 5 minutes each time.
e. Resuspend cells with serum-free cell culture medium or PBS. Observation by fluorescence microscopy or flow cytometry.
2.2 Adherent cells
a. Culture adherent cells on sterile coverslips.
b. Remove the coverslip from the medium and aspirate excess medium.
c. Add 100 μL of working solution, gently shake it to completely cover the cells,and then incubate at room temperature for 5-30 minutes.
d. Wash twice with medium, 5 minutes each time. Observation by fluorescence microscopy or flow cytometry.
Note: Protect from light

实验方案

计算器摩尔计算器总药量计算器工作液计算器
储存液制备
- 1 mg
- 5 mg
- 10 mg
- 1 mM
- 1.3163 mL
- 6.5815 mL
- 13.1631 mL
- 5 mM
- 0.2633 mL
- 1.3163 mL
- 2.6326 mL
- 10 mM
- 0.1316 mL
- 0.6582 mL
- 1.3163 mL

技术信息

CAS号	1003197-00-9	储存条件	粉末液体 -20°C:3-6 月 -80°C:12月
分子式	C43H43IN3P	运输	蓝冰
分子量	759.70	别名	MitoHE;Mito-hydroethidine

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