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CAS No.: 300586-90-7

OAC1 Catalog No. CSN15956

Synonyms: BAS 00287861

OAC1 is an Oct-4 activator used to enhance and accelerates iPSC reprogramming.

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技术信息

生物活性

描述Oct4 is a key regulator for ESC (embryonic stem cell) pluripotency. Reduced expression of Oct4 results in differentiation of ESCs into trophectodermal cells, and ovexpression of Oct4 leads to differentiation of ESCs along the mesodermal and primitive endodermal lineages. OAC1 (Oct4-activating compound 1), was found to activate both Oct4 and Nanog promoter-driven luciferase reporter genes. By counting the number of clones with ESC-like morphology at day 18, it was found that both OAC1 enhanced the 4F (a transcription factor quartet)-induced reprogramming efficiency considerably. The number of colonies with ESC-like morphology increased two fold or more in 4F plus OAC (4F+OAC) treatment, compared with the 4F treatment alone. Furthermore, addition of OAC1 to the reprogramming mixture considerably accelerated the appearance of iPSC-like colonies. Treatment of compound OAC1 started on day 1 and lasted for 7 d. Many GFP⁺ single cells were seen in treated cells at day 2 and the GFP⁺ colonies started to appear at day 3. At day 5, a more than threefold increase in the number of GFP⁺ colonies was detected in OAC1-treated, 4F-transduced cells (4F+OAC1), compared with vehicle-treated, 4F-transduced cells. At day 8, an approximately fourfold increase in the number of GFP⁺ colonies were observed in 4F+OAC1-treated cells (2.75% reprogramming efficiency), compared with 4F-treated cells (0.68% efficiency)^[2].
参考文献

[1] Li W, Tian E, et al. Identification of Oct4-activating compounds that enhance reprogramming efficiency. Proc Natl Acad Sci U S A. 2012 Dec 18;109(51):20853-8.
[2] Li W, Tian E, Chen ZX, et al. Identification of Oct4-activating compounds that enhance reprogramming efficiency. Proc Natl Acad Sci U S A. 2012;109(51):20853‐20858

实验方案

计算器摩尔计算器总药量计算器工作液计算器
储存液制备
- 1 mg
- 5 mg
- 10 mg
- 1 mM
- 4.2148 mL
- 21.0739 mL
- 42.1479 mL
- 5 mM
- 0.8430 mL
- 4.2148 mL
- 8.4296 mL
- 10 mM
- 0.4215 mL
- 2.1074 mL
- 4.2148 mL

技术信息

CAS号

300586-90-7

储存条件

粉末: Keep in dark place,Inert atmosphere,Store in freezer, under -20°C

液体: -20°C:3-6 月 -80°C:12月

分子式

C14H11N3O

运输

蓝冰

分子量

237.26

别名

BAS 00287861

溶解度

DMSO	440.0 mg/mL (1854.5 mM)
Water	insoluble

动物实验配方

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