|3-(3,4-二羟基苯基)丙烯酸苯乙酯|CsnPharm

CAS No.: 104594-70-9

Phenethyl caffeate/CAPE (咖啡酸苯乙酯) Catalog No. CSN10538

Synonyms: CAPE;Phenylethyl Caffeate;Caffeic Acid phenylethyl ester

Caffeic acid phenethyl ester, a natural product isolated and purified from the barks of Cinnamomum cassia Presl, is a potent and specific inhibitor of NF-κB activation, and also displays antioxidant, immunomodulatory and antiinflammatory activities.

规格	价格	促销价格	库存	数量

400-920-2911

sales@csnpharm.cn

tech@csnpharm.cn

加入购物车大规格询价

加入收藏

产品咨询

400-920-2911

sales@csnpharm.cn

tech@csnpharm.cn

纯度 & 质量文件批次:

靶点选择性

生物活性

实验方案

技术信息

生物活性

描述NF-κB is a transcription factor with heterodimer or homodimer form of rel family proteins including RelA (p65), RelB, cRel, p50 and p52. Caffeic Acid Phenethyl Ester (CAPE) is an NF-κB inhibitor isolated from propolis. Treatment with CAPE at concentration ranging in 20-40μM for 2h could dose-dependently inhibit phosphorylation of IκBα and subsequently nuclear translocation of NF-κB (present as p65) induced by LPS in hepatic stellate cells. The increased nitrite production and the transcription and protein synthesis of MCP-1, IL-6 and iNOS induced by LPS could be significantly reduced by CAPE at concentration ranging in 5-40μM in a concentration-dependent manner in the same cells. Similarly, TNF-activated binding of NF-κB to DNA, phosphorylation of IκBα (within 30min) and p65 nuclear translocation could be observed in U937 cells pre-treated with 25μg/ml CAPE. Oral administration of 30mg/kg of CAPE daily for 4 weeks could improve the glucose sensitivity associated with the improvement in insulin-stimulated phosphorylation of the insulin receptor substrate-2 and an increase in Akt phosphorylation, promote amelioration in hepaticsteatosis, as well as reduced the induction of the inflammatory pathway, including JNK, NF-κB and COX-2 pathway, in high-fat-diet mice.
细胞研究

Cell Data

Cell Lines	Concentration	Assay Type	Time	Activity Description	Data Sources
human A549 cells	-	Cytotoxic assay	72 h	Cytotoxicity against human A549 cells after 72 hrs by MTT assay, IC50=26.8 μM	20673727
human BCG823 cells	-	Cytotoxic assay	-	Cytotoxicity against human BCG823 cells by MTT assay, IC50=44.6 μM	18952420
human Bel7402 cells	-	Cytotoxic assay	-	Cytotoxicity against human Bel7402 cells by MTT assay, IC50=5.5 μM	18952420

动物研究

剂量

Rat: 3 mg/kg, 6 mg/kg^[3] (i.p.); 1 mg/kg - 20 mg/kg^[4] (i.v.); 20 μg/kg/day^[5] (p.o.) Pups: 40 mg/kg^[6] (i.p.)

给药途径

i.p., i.v., p.o.

动物药代数据

Animal	Rats^[7]
Dose	20 mg/kg
Administration	i.v.
CL_t	148.2 ± 70.4 ml/min/kg
T_1/2	28.7 ± 9.1 min
B	1.071 ± 0.926 μg/ml
α	0.468 ± 0.038 min^-1
V_z	5784 ± 2579 min/kg
A	53.6 ± 15.6 μg/ml
AUC_∞	160.5 ± 74.4 μg·min/ml
β	0.027 ± 0.011 min^-1

临床研究
NCT号适应症或疾病临床期招募状态预计完成时间地点
NCT02744703 Matrix Metalloproteinase Inhib... more >>itors Composite Resins Collapse << Not Applicable Completed - -
参考文献

NCT号	适应症或疾病	临床期	招募状态	预计完成时间	地点
NCT02744703	Matrix Metalloproteinase Inhib... more >>itors Composite Resins Collapse <<	Not Applicable	Completed	-	-

实验方案

计算器摩尔计算器总药量计算器工作液计算器
储存液制备
- 1 mg
- 5 mg
- 10 mg
- 1 mM
- 3.5173 mL
- 17.5864 mL
- 35.1729 mL
- 5 mM
- 0.7035 mL
- 3.5173 mL
- 7.0346 mL
- 10 mM
- 0.3517 mL
- 1.7586 mL
- 3.5173 mL

技术信息

CAS号

104594-70-9

储存条件

粉末: Keep in dark place,Sealed in dry,Store in freezer, under -20°C

液体: -20°C:3-6 月 -80°C:12月

分子式

C17H16O4

运输

蓝冰

分子量

284.31

别名

CAPE;Phenylethyl Caffeate;Caffeic Acid phenylethyl ester;2-Phenylethyl Caffeate;β-Phenylethyl Caffeate;Caffeic Acid Phenethyl Ester;BAF-IN-C09;咖啡酸苯乙酯

溶解度

DMSO	54.0 mg/mL (189.9 mM)
Water	Insoluble
Ethanol	55.0 mg/mL (193.5 mM)

动物实验配方

大规格询价

号为必填项

姓名: 邮箱: 手机号:

留言:

提交

Cell Lines	Concentration	Assay Type	Time	Activity Description	Data Sources

human A549 cells	-	Cytotoxic assay	72 h	Cytotoxicity against human A549 cells after 72 hrs by MTT assay, IC50=26.8 μM	20673727
human BCG823 cells	-	Cytotoxic assay	-	Cytotoxicity against human BCG823 cells by MTT assay, IC50=44.6 μM	18952420
human Bel7402 cells	-	Cytotoxic assay	-	Cytotoxicity against human Bel7402 cells by MTT assay, IC50=5.5 μM	18952420
human Bel7404 cells	-	Cytotoxic assay	72 h	Cytotoxicity against human Bel7404 cells after 72 hrs by MTT assay, IC50=14.5 μM	20673727
human Bewo cells	-	Function assay	-	Antitumor activity against human Bewo cells assessed as inhibition of cell growth by MTT assay, IC50=2.96 μM	25160837
human BGC823 cells	-	Cytotoxic assay	72 h	Cytotoxicity against human BGC823 cells after 72 hrs by MTT assay, IC50=19.3 μM	20673727
human ECA109 cells	-	Cytotoxic assay	72 h	Cytotoxicity against human ECA109 cells after 72 hrs by MTT assay, IC50=42.6 μM	20673727
human HeLa cells	-	Proliferation assay	72 h	Antiproliferative activity against human HeLa cells after 72 hrs by MTT assay, EC50=2.36 μM	12027739
human HeLa cells	-	Function assay	-	Antitumor activity against human HeLa cells assessed as inhibition of cell growth by MTT assay, IC50=37.98 μM	25160837
human HepG2 cells	-	Function assay	-	Antitumor activity against human HepG2 cells assessed as inhibition of cell growth by MTT assay, IC50=40.87 μM	25160837
human HL60 cells	-	Cytotoxic assay	72 h	Cytotoxicity against human HL60 cells after 72 hrs by MTT assay, IC50=9.8 μM	20673727
human HO8910 cells	-	Cytotoxic assay	72 h	Cytotoxicity against human HO8910 cells after 72 hrs by MTT assay, IC50=25.5 μM	20673727
human HT1080 cells	-	Proliferation assay	72 h	Antiproliferative activity against human HT1080 cells after 72 hrs by MTT assay, EC50=9.5 μM	12027739
human K562 cells	-	Cytotoxic assay	72 h	Cytotoxicity against human K562 cells after 72 hrs by MTT assay, IC50=46 μM	20673727
human KB cells	-	Cytotoxic assay	72 h	Cytotoxicity against human KB cells after 72 hrs by MTT assay, IC50=45.2 μM	20673727
human LNCAP cells	-	Function assay	24 h	Antiandrogenic activity in human LNCAP cells assessed as reduction in DHT-stimulated PSA release after 24 hrs by ELISA, IC50=6.2 nM	24080105
human LNCAP cells	-	Cytotoxic assay	24 h	Cytotoxicity against human LNCAP cells assessed as reduction in cell viability after 24 hrs by WST-1 assay, IC50=33.7 μM	24080105
human LS 174T cells	-	Cytotoxic assay	72 h	Cytotoxicity against human LS 174T cells after 72 hrs by MTT assay, IC60=9.9 μM	20673727
human MCF7 cells	-	Cytotoxic assay	-	Cytotoxicity against human MCF7 cells by MTT assay, IC50=26.7 μM	18952420
human SGC7901 cells	-	Function assay	-	Antitumor activity against human SGC7901 cells assessed as inhibition of cell growth by MTT assay, IC50=14.26 μM	25160837
HUVEC cells	-	Cytotoxic assay	18 h	Cytoprotective activity against H2O2-induced oxidative stress in HUVEC cells after 18 hrs by Cell-Titer Blue assay	20598894
mouse 26-L5 cells	-	Proliferation assay	72 h	Antiproliferative activity against mouse 26-L5 cells after 72 hrs by MTT assay, EC50=0.3 μM	12027739
mouse B16-BL6 cells	-	Proliferation assay	72 h	Antiproliferative activity against mouse B16-BL6 cells after 72 hrs by MTT assay, EC50=6.79 μM	12027739
mouse BV2 cells	-	Function assay	24 h	Inhibition of nitric oxide production in LPS-stimulated mouse BV2 cells treated 3 hrs before LPS addition measured after 24 hrs by Griess assay, IC50=6.4 μM	23870700
mouse J774.1 cells	-	Function assay	24 h	Inhibition of LPS-induced NO production in mouse J774.1 cells after 24 hrs by Griess reagent assay, IC50=4.8 μM	15974608
mouse RAW264.7 cells	-	Function assay	24 h	Inhibition of LPS-induced nitric oxide production in mouse RAW264.7 cells assessed as nitrite accumulation administered 1 hr before LPS challenge and measured after 24 hrs by Griess reagent assay, EC50=0.193 μM	18667320
rat PC12 cells	2.5 μM	Function assay	3 h	Neuroprotective activity against H2O2-induced damage in rat PC12 cells assessed as cell viability at 2.5 uM preincubated for 3 hrs followed by H2O2 challenge measured after 5 hrs by MTT assay	24697335
rat VSMC	5 μM	Function assay	30 mins	Decrease in intracellular ROS level in PDGF-stimulated rat VSMC at 5 uM pretreated with compound for 30 mins measured after up to 23 hrs of post stimulation using H2DCF by flow cytometric analysis	21265554

Phenethyl caffeate/CAPE (咖啡酸苯乙酯) Catalog No. CSN10538

靶点选择性

请登录您的专属账户

确认信息