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CAS No.: 30636-90-9

Protopanaxdiol/原人参二醇 Catalog No. CSN18195

Synonyms: 20-Epiprotopanaxadiol;20(S)-APPD;20(S)-Protopanaxadiol

(20S)-Protopanaxadiol is an aglycon metabolic derivative of the protopanaxadiol-type ginseng saponin and an apoptosis inducer.

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技术信息

生物活性

描述(20S)-Protopanaxadiol (20-Epiprotopanaxadiol) is an aglycon metabolic derivative of the protopanaxadiol-type ginseng saponin-apoptosis inducer. For the SF188 cells, (20S)-Protopanaxadiol activated caspases-3, -8, -7, and -9 within 3 h and induced rapid apoptosis, which could be partially inhibited by a general caspase blocker and completely abolished when the caspase blocker was used in combination with an antioxidant^[3]. 20S-protopanaxadiol (PPD) showed the most potent cytotoxic activity in both LX-2 cells and primary activated HSCs (hepatic stellate cells) . In addition, 20S-PPD concentration-dependently increased the phosphorylation of AMPK, and compound C prevented 20S-PPD-induced cytotoxicity and mitochondrial dysfunction. Moreover, 20S-PPD increased the phosphorylation of liver kinase B1 (LKB1), an upstream kinase of AMPK. Likewise, transfection of LX-2 cells with LKB1 siRNA reduced the cytotoxic effect of 20S-PPD^[4]. PPD (>30 μM) suppressed cell proliferation by arresting cell cycle in G0/1 phase and triggering cells apoptosis as shown by cell viability assay, flow cytometry analysis and colony formation assay, whereas lower dose of PPD (<20 μM) decreased metastatic potential of MDA-MB-231 and SUM159 cells through direct inhibition of cell adhesion, motility and invasiveness^[5].
细胞研究

Cell Data

Cell Lines	Concentration	Assay Type	Time	Activity Description	Data Sources
COLO205 cells	-	Cytotoxicity assay	72 h	Cytotoxicity against human COLO205 cells after 72 hrs by MTT assay	21216145
Hep3B cells	-	Cytotoxicity assay	72 h	Cytotoxicity against human Hep3B cells after 72 hrs by MTT assay	22342101
HepG2 cells	-	Cytotoxicity assay	72 h	Cytotoxicity against human HepG2 cells after 72 hrs by MTT assay	21216145

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参考文献

实验方案

计算器摩尔计算器总药量计算器工作液计算器
储存液制备
- 1 mg
- 5 mg
- 10 mg
- 1 mM
- 2.1705 mL
- 10.8523 mL
- 21.7046 mL
- 5 mM
- 0.4341 mL
- 2.1705 mL
- 4.3409 mL
- 10 mM
- 0.2170 mL
- 1.0852 mL
- 2.1705 mL

技术信息

CAS号

30636-90-9

储存条件

粉末: Sealed in dry,2-8°C

液体: -20°C:3-6 月 -80°C:12月

分子式

C30H52O3

运输

蓝冰

分子量

460.73

别名

20-Epiprotopanaxadiol;20(S)-APPD;20(S)-Protopanaxadiol;20 (S)-原人参二醇;原人参二醇

溶解度

DMSO	92.0 mg/mL (199.7 mM)
Water	Insoluble
Ethanol	32.0 mg/mL (69.5 mM)

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Cell Lines	Concentration	Assay Type	Time	Activity Description	Data Sources

COLO205 cells	-	Cytotoxicity assay	72 h	Cytotoxicity against human COLO205 cells after 72 hrs by MTT assay	21216145
Hep3B cells	-	Cytotoxicity assay	72 h	Cytotoxicity against human Hep3B cells after 72 hrs by MTT assay	22342101
HepG2 cells	-	Cytotoxicity assay	72 h	Cytotoxicity against human HepG2 cells after 72 hrs by MTT assay	21216145
HepG2 cells	-	Cytotoxicity assay	48 h	Cytotoxicity against human HepG2 cells after 48 hrs by MTT assay	26420067
HL60 cells	-	Cytotoxicity assay	72 h	Cytotoxicity against human HL60 cells after 72 hrs by MTT assay	21216145
HL60 cells	-	Cytotoxicity assay	48 h	Cytotoxicity against human HL60 cells after 48 hrs by MTT assay	26420067
LLC-PK1	-	Cytotoxicity assay	4 h	Cytotoxicity against pig LLC-PK1 cells after 4 hrs by MTT assay	25453798
MCF7 cells	-	Cytotoxicity assay	72 h	Cytotoxicity against human MCF7 cells after 72 hrs by MTT assay	21216145
MDA-MB-231 cells	2.5-5.8 μM	Function assay	24 h	Induction of apoptosis in human MDA-MB-231 cells assessed as increase in expression of cleaved PARP at 2.5 to 5.8 uM after 24 hrs by SDS-polyactylamide gel electrophoresis	25453798
MDA-MB-231 cells	-	Proliferation assay	4 h	Antiproliferative activity against human MDA-MB-231 cells after 4 hrs by MTT assay	25453798
SF188 cells	-	Cytotoxicity assay	24 h	Cytotoxicity against human SF188 cells after 24 hrs	17261067
SF188 cells	108.4 μM	Function assay	4 h	Induction of autophagy in human SF188 cells by MDC staining at 108.4 uM after 4 hrs	17261067
SF188 cells	108.4 μM	Function assay	-	Activation of caspase 3 in human SF188 cells at 108.4 uM	17261067
SF188 cells	-	Function assay	2 h	Increase in superoxide anion level in human SF188 cells after 2 hrs	17261067
SF188 cells	108.4 μM	Function assay	-	Activation of caspase 7 in human SF188 cells at 108.4 uM	17261067
SF188 cells	43.4 μM	Function assay	4 h	Induction of autophagy in human SF188 cells by MDC staining at 43.4 uM after 4 hrs	17261067
U87 cell	-	Cytotoxicity assay	72 h	Cytotoxicity against human U87 cells after 72 hrs by MTT assay	22342101
U87MG cells	-	Cytotoxicity assay	24 h	Cytotoxicity against human U87MG cells after 24 hrs	17261067
U87MG cells	-	Function assay	2 h	Increase in superoxide anion level in human U87MG cells after 2 hrs	17261067
U87MG cells	43.4 μM	Function assay	4 h	Induction of autophagy in human U87MG cells by MDC staining at 43.4 uM after 4 hrs	17261067

Protopanaxdiol/原人参二醇 Catalog No. CSN18195

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