|7-(((2S,3R,4S,5R,6R)-3,4,5-三羟基-6-(羟甲基)四氢-2H-吡喃-2-基)氧基)-3H-吩恶嗪-3-酮|CsnPharm

CAS No.: 95079-19-9

Resorufin β-D-Galactopyranoside/试卤灵-β-D-吡喃半乳糖苷 Catalog No. CSN32727

Synonyms: Resorufinbeta-d-galactopyranoside;Resorufin galactopyranoside

Resorufin β-D-galactopyranoside is a fluorogenic substrate for β-galactosidase. Resorufin displays excitation/emission maxima of 570/580 nm, respectively.

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技术信息

生物活性

描述Resorufin β-D-galactopyranoside is a fluorogenic substrate for β-galactosidase. Resorufin displays excitation/emission maxima of 570/580 nm, respectively. Resorufin-β-d-galactopyranoside (RGP), providing the orange fluorescing resorufin after enzymatic cleavage, was introduced as pYES substrate to determine EAC (estrogen active compounds). The application of the RGP-pYES on waste water influent and effluent samples showed the clear detection of EAC without interferences^[1]. Planar yeast estrogen screen (pYES) with the substrate resorufin-β-d-galactopyranoside (RGP) successfully was applied for the detection of EAC in ethanolic extracts of hops pellet samples^[2]. Using beta-galactosidase and its substrate, resorufin-beta-D-galactopyranoside, as the model system of the enzyme reaction. With a single experiment on the chip to determine the key parameters for the enzyme kinetics, K(m) and k(cat), and evaluated the effect of inhibitor concentrations on the reaction rates^[3].
参考文献

[1] Schick D, Schwack W. Planar yeast estrogen screen with resorufin-β-d-galactopyranoside as substrate. J Chromatogr A. 2017 May 12;1497:155-163
[2] Schick D, Schwack W. Detection of estrogen active compounds in hops by planar yeast estrogen screen. J Chromatogr A. 2018 Jan 12;1532:191-197
[3] Jambovane S, Duin EC, Kim SK, Hong JW. Determination of kinetic parameters, Km and kcat, with a single experiment on a chip. Anal Chem. 2009 May 1;81(9):3239-45

实验方案

计算器摩尔计算器总药量计算器工作液计算器
储存液制备
- 1 mg
- 5 mg
- 10 mg
- 1 mM
- 2.6643 mL
- 13.3216 mL
- 26.6433 mL
- 5 mM
- 0.5329 mL
- 2.6643 mL
- 5.3287 mL
- 10 mM
- 0.2664 mL
- 1.3322 mL
- 2.6643 mL

技术信息

CAS号	95079-19-9	储存条件	粉末 Keep in dark place,Inert atmosphere,Store in freezer, under -20°C 液体 -20°C:3-6 月 -80°C:12月
分子式	C18H17NO8	运输	蓝冰
分子量	375.33	别名	Resorufinbeta-d-galactopyranoside;Resorufin galactopyranoside

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Resorufin β-D-Galactopyranoside/试卤灵-β-D-吡喃半乳糖苷 Catalog No. CSN32727

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