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CAS No.: 260553-97-7

TM-25659 Catalog No. CSN23679

Synonyms: TM-25659

TM-25659 is a modulator of TAZ with anti-osteoporotic and anti-obesity effects.

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靶点选择性

生物活性

实验方案

技术信息

生物活性

描述The protein transcriptional co-activator with PDZ-binding motif (TAZ), also referred to as WW domain-containing transcriptional co-regulator (WWTR1), was originally cloned as an interacting protein of the 14-3-3 protein that is involved in the control of cell cycle progression, cell differentiation and apoptosis through interaction with phosphorylated signalling molecules. TM-25659 acts as a TAZ modulator that enhances TAZ expression in the nucleus. Treatment of COS-7 cells with TM-25659 for 30 min increased GFP (green fluorescence protein)-TAZ intensities in a dose-dependent manner. The nuclear level of endogenous TAZ was increased by TM-25659 which was confirmed by immunofluorescence staining with anti-TAZ antibody. In 3T3-L1 cells, TM-25659 treatment for 2 hrs significantly suppressed the normal adipocyte differentiation and lipid droplet formation. As a result of attenuated adipogenic differentiation by TM-25659, the increased levels of PPARγ in differentiated adipocytes were decreased by TM-25659 in a concentration-dependent manner, whereas TAZ expression was substantially elevated. In addition, the adipogenic marker aP2 and adiponectin expression were further decreased by TM-25659. These results demonstrate that TM-25659 acted as a suppressor of PPARγ-dependent adipocyte differentiation. Moreover, the expression levels of osteogenic markers, such as osteocalcin, ALP and osterix were significantly increased by treatment with TM-25659, even at a concentration of 2 µM. In vivo, body weight increase caused by the HFD (high fat diet) was substantially diminished in the TM-25659-treated group compared with the vehicle-treated group^[1].
参考文献

[1] Jang EJ, Jeong H, Kang JO, et al. TM-25659 enhances osteogenic differentiation and suppresses adipogenic differentiation by modulating the transcriptional co-activator TAZ. Br J Pharmacol. 2012;165(5):1584‐1594

实验方案

计算器摩尔计算器总药量计算器工作液计算器
储存液制备
- 1 mg
- 5 mg
- 10 mg
- 1 mM
- 1.9976 mL
- 9.9880 mL
- 19.9760 mL
- 5 mM
- 0.3995 mL
- 1.9976 mL
- 3.9952 mL
- 10 mM
- 0.1998 mL
- 0.9988 mL
- 1.9976 mL

技术信息

CAS号

260553-97-7

储存条件

粉末: Inert atmosphere,2-8°C

液体: -20°C:3-6 月 -80°C:12月

分子式

C30H28N8

运输

蓝冰

分子量

500.60

别名

TM-25659

溶解度

DMSO	275.0 mg/mL (549.3 mM)
Water	insoluble

动物实验配方

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