1062169-56-5|WYE-354|CsnPharm

CAS No.: 1062169-56-5

WYE-354 Catalog No. CSN16829

Synonyms: Wye-354

WYE-354 is a potent, specific and ATP-competitive inhibitor of mTOR with IC50 of 5 nM, blocks mTORC1/P-S6K (T389) and mTORC2/P-AKT (S473) not P-AKT (T308), selective for mTOR than PI3Kα (> 100-fold) and PI3Kγ (> 500-fold).

规格	价格	促销价格	库存	数量

400-920-2911

sales@csnpharm.cn

tech@csnpharm.cn

加入购物车大规格询价

加入收藏

产品咨询

400-920-2911

sales@csnpharm.cn

tech@csnpharm.cn

纯度 & 质量文件批次:

靶点选择性

生物活性

实验方案

技术信息

生物活性

描述The mammalian target of rapamycin (mTOR) is centrally involved in cell growth, metabolism, and angiogenesis^[3]. WYE-354 is an ATP-competitive mTOR inhibitor with an IC₅₀ of 5 nM. WYE-354 also inhibits PI3Kα and PI3Kγ with IC₅₀s of 1.89 μM and 7.37 μM, respectively ^[3]. In vitro assays showed WYE-354 significantly reduced cell viability, migration and invasion and phospho-P70S6K expression in GBC cells^[4]. When injected into tumor-bearing mice, WYE-354 inhibited mTORC1 and mTORC2 and displayed robust antitumor activity in PTEN-null tumors ^[3]. Mice harboring subcutaneous gallbladder tumors, treated with WYE-354, exhibited a significant reduction in tumor mass. A short-term treatment with a higher dose of WYE-354 decreased the tumor size by 68.6% and 52.4% , in mice harboring G-415 or TGBC-2TKB tumors, respectively, compared to the control group^[4].
细胞研究

Cell Data

Cell Lines	Concentration	Assay Type	Time	Activity Description	Data Sources
HEK293 cells	-	Function assay	-	Inhibition of flag-tagged truncated human mTOR (1360-2549) expressed in HEK293 cells by DELFIA assay, IC50=0.0043 μM	19645448
LNCAP cells	-	Cytotoxicity assay	3 days	Cytotoxicity against human LNCAP cells after 3 days by MTS assay, IC50=0.355 μM	19645448

参考文献

[1] Dormond-Meuwly A, Roulin D, et al. The inhibition of MAPK potentiates the anti-angiogenic efficacy of mTOR inhibitors. Biochem Biophys Res Commun. 2011 Apr 22;407(4):714-9.
[2] Yu K, Toral-Barza L, et al. Biochemical, cellular, and in vivo activity of novel ATP-competitive and selective inhibitors of the mammalian target of rapamycin. Cancer Res. 2009 Aug 1;69(15):6232-40.
[3] Yu K, Toral-Barza L, Shi C, et al. Biochemical, cellular, and in vivo activity of novel ATP-competitive and selective inhibitors of the mammalian target of rapamycin. Cancer Res. 2009;69(15):6232–6240.
[4] Weber H, Leal P, Stein S, et al. Rapamycin and WYE-354 suppress human gallbladder cancer xenografts in mice. Oncotarget. 2015;6(31):31877–31888.

实验方案

计算器摩尔计算器总药量计算器工作液计算器
储存液制备
- 1 mg
- 5 mg
- 10 mg
- 1 mM
- 2.0180 mL
- 10.0902 mL
- 20.1804 mL
- 5 mM
- 0.4036 mL
- 2.0180 mL
- 4.0361 mL
- 10 mM
- 0.2018 mL
- 1.0090 mL
- 2.0180 mL

技术信息

CAS号

1062169-56-5

储存条件

粉末: Sealed in dry,Store in freezer, under -20°C

液体: -20°C:3-6 月 -80°C:12月

分子式

C24H29N7O5

运输

蓝冰

分子量

495.53

别名

Wye-354

溶解度

DMSO	28.75 mg/mL (58.0 mM)
Water	insoluble

动物实验配方

4% DMSO+30% PEG 300+5% Tween 80+water

5 mg/mL

大规格询价

号为必填项

姓名: 邮箱: 手机号:

留言:

提交

WYE-354 Catalog No. CSN16829

靶点选择性

请登录您的专属账户

确认信息