Safranine T （Safranin O） is a metachromatic, cationic dye.

Store at room temperature.

Protocol for Safranine T staining of cartilage After conventional dewaxing to water, the cartilage sections are dipped in 1% Safranine T for 30 min and subsequently in 0.5% Fast Green solution for 1 min. They then undergo conventional processing and were mounted. The sections are imaged using an automatic quantitative pathological imaging system. The cartilage and mucin will be stained orange to red. {{Shi X, Yu W, Wang T, Battulga O, Wang C, Shu Q, Yang X, Liu C, Guo C. Electroacupuncture alleviates cartilage degradation: Improvement in cartilage biomechanics via pain relief and potentiation of muscle function in a rabbit model of knee osteoarthritis. Biomed Pharmacother. 2020 Mar;123:109724.| https://pubmed.ncbi.nlm.nih.gov/31918209/}} Protocol for Safranine T in Gram staining 1. Prepare and heat-fix bacterial smears. 2. Flood the slides crystal violet for 1 minute. 3. Pour off excess dye and wash gently in tap water and drain the slide against a paper towel. 4. Expose the smears to

Safranine T （Safranin O） is a metachromatic, cationic dye. It is used as a counterstain in Gram staining. The stain colors Gram-negative bacteria pink to red and has no effect on Gram-positive bacteria. It is also used to assess glycosaminoglycans (GAGs) in cartilage. The dye imparts an intense red to GAGs. It easily binds to polyanions.